Journal: Kidney international

Article Title: Peritubular capillaries are rarefied in congenital nephrotic syndrome of the Finnish type.

doi: 10.1038/ki.2009.41

Figure Lengend Snippet: Figure 4 | The expression of adhesion molecules and their ligands in peritubular space of NPHS1 kidneys. (a) The staining intensity for ICAM-1 was 1.5 times (Po0.01) higher in NPHS1 kidneys as compared with control group 1, whereas its expression was equal to control group 2. (b) The expression of P-selectin was modest, although higher ( 2.1) than in controls. (c–e) Peritubular expression of VCAM-1, E-selectin (ELAM-1), and CD29 (b1-integrin) was very modest. (f–h) The expression levels of LFA-1 (ligand for ICAM-1), PSGL-1 (ligand for P-selectin), and VLA-4 (ligand for VCAM-1) were higher in NPHS1 kidneys as compared with control groups 1 and 2. (j and k) The results are presented as an average of PTC-positive foci per visual field. (Original magnification 400) **Po0.01.

Article Snippet: Antibodies for immunoperoxidase stainings included mouse monoclonal antibodies against CD29 (Visionbiosystems novocastra, Newcastle upon Tyne, UK), CD61 (Dako Cytomation), CD34 (Dako Cytomation), collagen I (Acris Antibodies, Hiddenhausen, Germany), endothelial leukocyte adhesion molecule (ELAM-1, Eselectin, CD62E) (R&D Systems, Minneapolis, MN, USA), human SMA (Dako Cytomation), intercellular adhesion molecule-1 (ICAM-1, CD54) (R&D Systems), leukocyte function antigen-1 (LFA-1, CD11a) (Dako Cytomation), platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) (Dako Cytomation), P-selectin glycoprotein ligand (PSGL-1, CD162) (Santa Cruz Biotechnology Inc.), Sialyl-LewisX (sLEX, CD15s) (BD Pharmingen, San Jose, CA, USA), vascular cell adhesion molecule-1 (VCAM-1, CD106) (R&D Systems), very late antigen-4 (VLA-4, CD49d) (Serotec, Oxford, UK).

Techniques: Expressing, Staining, Control